Abstract
In the present studies the regulation of cAMP by the opioid peptide DADLE was examined in mouse striatum and spinal cord. For comparison, DADLE inhibition of cAMP was examined in the rat striatum. In all three tissue preparations (mouse and rat striatum and mouse spinal cord) DADLE inhibited cAMP in a dose-dependent manner. Mouse and rat striatum were equally sensitive to DADLE inhibition of cAMP, while mouse cord was less sensitive. Naloxone reversed the effect of DADLE in the rat striatum. Conversely, the DADLE effect was not naloxone reversible in the mouse striatum and spinal cord. In addition, naloxone by itself reduced basal cAMP dose-dependently in the mouse striatum. Chronic in vivo naltrexone treatment increased the in vitro potency of DADLE to inhibit cAMP in the rat striatum. However, naltrexone treatment did not increase sensitivity to DADLE in the mouse striatum and mouse spinal cord. Overall, opioid effects on cAMP in mouse striatum and spinal cord differ from that measured in rat striatum.
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