Abstract
Abstract In 1965, the “two gene-one polypeptide” theory for the synthesis of antibody chains was first proposed (1). Since then, amino acid sequence studies and genetic analyses of the variable (V)2 and constant (C) regions of light (L) and heavy (H) chains have been consistent with this hypothesis (2). A direct demonstration of the existence of separate genetic units was achieved in 1977 (3), when it was shown that the DNA segments (exon DNA) coding for V and C regions of mouse L chains (λ type) are separated from one another by a large DNA segment (intron or intervening sequence or IVS) that is missing from the mRNA that serves as the template for the L chain. In addition, in DNA derived from embryonic cells, the V gene is shorter than expected (4, 5), ending at the codon specifying position 98 instead of 112 commonly accepted at the VC joining region.
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