Abstract
Creeping bentgrass commonly is grown on golf courses in the United States. In the fall of 1998, symptoms of a new disease were observed on bentgrass putting greens in three states. The causal agent was determined to be an undescribed species of Ophiosphaerella based on morphological character- istics. This was supported by sequence analysis of the complete ITS region of the rDNA. A new species, Ophiosphaerella agrostis, is described based on these morphological and molecular analyses. Intraspecific genetic variation was assessed using amplified frag- ment length polymorphism DNA fingerprinting of 17 isolates collected from nine different locations. A 90% similarity was found among the isolates.
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