Abstract
A study was undertaken of the effects of varying those parameters that most affect analyses by capillary zone electrophoresis (CZE), electrolyte buffer, concentration of electrolyte, pH, voltage, temperature, and diameter of capillary, in respect to the following aromatic diamidines of therapeutic value: pentamidine, hydroxystilbamidine, propamidine, 6-amidino-2-(4-amidinophenyl) indole dilactate (DAPI), and stilbamidine. The data obtained permit the design of specific analytic methodology for each of these chemicals under differing conditions. As a demonstration, an analytical method is proposed in which all these chemicals are analyzed, simultaneously, in serum and urine under the following CZE conditions: citrate buffer 25 mM, pH = 3.75, T = 30°C, and V = 14 kV. The detection limits for the method for serum and urine are (μg/mL): pentamidine, 0.3; hydroxystilbamidine, 0.4; propamidine, 0.2; DAPI, 0.3; and stilbamidine, 0.3. Oasis HLB cartridges were used for prior treatment of both serum and urine samples.
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