Opening Hemichannels in Nonjunctional Membrane Stimulates Gap Junction Formation

Abstract

We studied gap junction formation in pairs of Xenopus laevis oocytes expressing connexins that form functional hemichannels and found no correlation between junctional conductance ( G j) and whole-cell hemichannel conductances ( G hemi) within the first few hours of pairing. However, opening hemichannels to a threshold current stimulated a rapid G j increase. Moreover, cx46 hemichannel current stimulated cx40 G j even though cx40 and cx46 do not form heteromeric or heterotypic gap junctions. Initial growth rate and final steady-state level of stimulated G j were proportional to the product of hemichannel conductances. External calcium affected the growth rate of stimulated G j but not the final steady-state value. Time constants of formation were short in low [Ca 2+] out (3 min in 200 μM Ca 2+) and long in high [Ca 2+] out (15 min in 1 mM Ca 2+), but in oocyte pairs pretreated with lectins to reduce steric hindrance imposed by large membrane glycoproteins the time constant was short and Ca 2+-independent. We suggest that hemichannel activity stimulates G j by collapsing the extracellular volume between membranes to allow the end-to-end binding between hemichannels. These studies suggest the possibility that functional hemichannels could trigger or enhance junctional formation in vivo in response to appropriate stimuli.