Open-source cell extension system assembled from laser-cut plates

Abstract

Abstract Biological response of cells to mechanical stimulation plays an important role in regulation of cell activity, and thus has been an attracting topic for researchers in the field of biomechanics for many years. Stretching is the most common type of the mechanical stimulus that has been used for in vitro experiments. The device for application of stretching to cells has commercially been available for more than three decades. Although these ready-made devices will bring a smooth start of research work, they cost several thousands to tens of thousands dollars for introduction. Therefore, this study proposes an open-source self-made cell extension system which can be built at one tenth to one hundredth of the price of commercial devices. To reduce the difficulty of machining, the device was designed to be assembled from acrylic plates fabricated only by using a laser cutter without machining such as drilling or screw thread cutting. All other mechanical elements and electrical components are purchased from web shops. The accuracy of the reciprocating motion was verified with the fabricated device and an elastic silicone container for cell culture.