Abstract
Membrane-based sampling systems encounter problems when sampling high molecular weight or highly lipophilic substances in the interstitial fluid. Open flow microperfusion (OFM) overcomes these problems by replacing the membrane with a steel mesh featuring macroscopic openings in combination with a peristaltic OFM pump in push/pull mode to achieve stable recovery of OFM samples. Unfiltered sampling results in a complete representation of the ISF for relative and absolute quantification in the target tissue. Current applications in adipose subcutaneous tissue (aOFM) and dermal tissue (dOFM) range from preclinical studies to clinical trials, and cover a wide range of substances from small ions to lipophilic topical drugs to large antibodies. The latest development in OFM has been designed for use in cerebral tissue (cOFM). Currently used in preclinical research, cOFM allows effective sampling in brain tissue with an intact blood–brain barrier. Future work will combine OFM with metabolomics for a more complete assessment of metabolic pathways.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
