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Abstract
A new approach for visual and colorimetric detection of glutathione (GSH) in an aqueous solution has been developed that is extremely simple and rapid. The designed biosensor SL1 used is simple to make and utilize, with the additional benefits of being cost-effective. By changes in absorption intensity as well as colorimetrically, it shows superb selectivity and ultrasensitivity towards GSH over other amino acids and biomolecules. Based on the information provided above, we developed an approach to determine GSH qualitatively and quantitatively using UV spectrophotometer, paper based test strips, and visually, respectively. A satisfactory linear relationship may be achieved from 0.26 to 52 μM with a detection limit (LOD) of 63 nM and detection quantitation (LOQ) 210 nM. Moreover, the high recovery rates for human blood serum samples demonstrated that the proposed colorimetric technique is practicable, straightforward, and ultrasensitive, with a prominent application in regular biosample analysis.
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