Abstract
The ultrastructural aspects of oocyte development in Dirofilaria immitis was studied with reference to the phylogenesis of mitochondria. Segments of ovaries from mature female worms were fixed in glutaraldehyde, postosmicated, dehydrated with ethanol, and embedded with Araldite 502. Thin sections from defined regions of ovaries were stained with uranyl acetate and lead citrate and viewed with a Hitachi HU-11A microscope. The method of Scarpelli, et al.. (1962), utilizing tetranitro-blue tetrazolium, was used for detecting the presence of succinic dehydrogenase and NADH-cytochrome c reductase.Cells from the germinal zone (within 5 cm. from the tip of ovary) are round and uniform in size. They have pseudppod-like processes (Fig. 1) by which they are interconnected. They possess a simple cytoplasmic organization and contain very few organelles (Fig. 2). The latter include the nucleus, nucleolus, lysosome-like granules, lipid droplets, scanty ribosomes, Golgi complex, and rudimentary mitochondria.
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