Abstract
1. Adult follicular fluid supplementation during in vitro maturation improves the developmental competence of prepubertal lamb oocytes Hao Tian, Kexiong Liu, Yumei Zhang, Qi Qi, Chunxin Wang, Hong Guan, Fengxiang Yan, Jian Hou Theriogenology CrossRef
Highlights
Trials undertaken to study the effects of follicular fluid on maturation and subsequent developmental potential in cattle comprised the inhibitory influence as well as stimulation
In an attempt to possibly simplify the procedure, we examine if bFF randomly collected from large follicles effectively supports oocyte maturation and preimplantation development
Comparison of cleavage dynamics and developmental competence of oocytes matured in the presence of bFF derived from three separate batches
Summary
Trials undertaken to study the effects of follicular fluid on maturation and subsequent developmental potential in cattle comprised the inhibitory influence as well as stimulation. Inhibitory effects on development appear to be related to small size of follicles from which bFF was obtained (e.g., Wang et al, 1999) and/ or to too high a concentration of bFF in the medium. Pooled bFF from small follicles improves blastocyst yield when used at 10-30% concentration, but inhibits development at 60% concentration (Kim et al, 1996). Carolan et al (1996) have shown that the action of follicular fluid on cytoplasmic maturation of cow oocytes varies with follicle quality. Several studies have shown that dominant follicles have a greater vascular bed and an increased gonadotrophin uptake compared with other antral follicles. Several studies have shown that dominant follicles have a greater vascular bed and an increased gonadotrophin uptake compared with other antral follicles. Acosta et al (2003) demonstrated that there is an increase in both vascular area and blood flow in the pre-ovulatory follicle during spontaneous ovulation
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