Abstract

1. The development and growth of the primary oocytes in the coelomic fluid are described. Oocytes progress from a packet stage to a single oocyte stage accompanied by vegetative growth of the germinal vesicle. Evidence presented indicates that the germinal vesicle breaks down and the oocyte reaches the first maturation division prior to shedding under natural conditions.2. Nuclear uptake of H3-thymidine is confined to the ovarian cells following the last oogonial division in the premeiotic phase. There is evidence that free coelomic oocyte packets incorporate H3-thymidine directly into the cytoplasm. The cytoplasmic label is removed by treatment with DNase.3. Short pulses of H3-uridine are taken up diffusely by the ovarian oocytes while small packets and single oocytes incorporate H3-uridine primarily in the nucleus and in the nucleolus. Extended exposure from 4 to 48 hours indicates that some of the nuclear uridine moves into the cytoplasm in the packet oocytes. Individual oocytes show strong nuclear lab...

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