Oocyte and somatic tyrosine tRNA genes in Xenopus laevis.

Abstract

Over a period of many months, Xenopus oocytes stockpile large quantities of tRNA for use during the first few hours of embryogenesis. To test the idea that these tRNAs are transcribed from one set of genes and that another set is used by somatic cells, we used synthetic oligonucleotides to analyze the sequence and steady-state levels of unspliced tyrosine tRNA precursors in Xenopus laevis oocytes, embryos, and cultured kidney cells. These analyses identify four kinds of tyrosine tRNA genes, two oocyte-type and two somatic-type, whose unspliced transcripts are distinguishable from one another by their different 5' leader and intervening sequences. The oocyte-type tyrosine tRNA precursors are present in oocytes, very abundant in gastrula embryos, but absent from postembryonic somatic cells. The somatic-type precursors are undetectable in oocytes but are found in gastrula and later stage embryos and in somatic cells. The major switch from oocyte-type to somatic-type transcripts occurs early during embryogenesis, between the midblastula transition and the onset of neurulation, but some oocyte-type precursors are also detectable in tadpoles.