Oocyst production and immunogenicity of Cryptosporidium muris (strain MCR) in mice.

Abstract

Three-week-old ICR SPF mice were orally inoculated with one of 5 doses ranging from 2 x 10(2) to 2 x 10(6) oocysts of Cryptosporidium muris (strain MCR) per mouse. Oocyst inoculation was directly proportional to the amount of oocysts shed and was inversely proportional to the period required for peak oocyst production and to the prepatent period. Peak oocyst production occurred between fifteen and thirty-one days with a patent period from 61 to 64 days. Three days after all mice stopped shedding oocysts, they were orally challenged with a single dose of 2 x 10(6) oocysts of the same species. Marked seroconversion for IgG antibody accompanied recovery from mice inoculated with 5 x 10(5) oocysts. Mice administered with carrageenan excreted a small number of oocysts for 49.0 days on the average after challenge inoculation (ACI) and control mice for 14.2 days in a dose-independent fashion. Just before challenge infection, phagocytic activity of peritoneal macrophages (M phi) and the number of peripheral M phi were dramatically decreased. Mild challenge infection implies that the immunogenicity of C. muris (strain MCR) is very strong, despite M phi blocker carrageenan administration.