Ontogeny of the fused tentacles in three species of ommastrephid squids (Cephalopoda, Ommastrephidae)

Abstract

Abstract. The tentacles of ommastrephid squids fuse during embryonic development and remain fused as they grow through hatching, but eventually separate to become two fully functional adult tentacles. The external anatomy of individuals at several post‐hatching ontogenetic stages of three species of ommastrephid squids (Ommastrephes bartramii, Sthenoteuthis oualaniensis, and Hyaloteuthis pelagica) was examined using scanning electron microscopy and morphometrics. The fusion of the transverse muscle mass of the tentacles was examined using light microscopy. Five ontogenetic stages of tentacle separation were defined based on landmark features such as the extent of the fusion and the presence of suckers or sucker buds at the distal tip. The total tentacle length and fused tentacle length reached a maximum when the dorsal mantle length (ML) equaled 3–4 mm (H. pelagica) or 4–6 mm (O. bartramii, S. oualaniensis), and then decreased with increasing ML. The average split length (measured from the base of the tentacles to the point of tentacle fusion) increased gradually with increasing ML, and the separate tentacle diameter was roughly half the diameter of the fused portion at all sizes. In all three species, separation of the fused tentacles began earlier in development (2–3‐mm ML) and was more advanced at smaller sizes than previously reported. The sizes presented here are conservative because excess epithelium at the location of the split may disguise the actual site of separation. Post‐separation tentacles were much shorter than the arms, and the carpal region appeared torn in 2 of the 4 specimens of S. oualaniensis examined. Finally, none of the original distal tip suckers were retained on the post‐separation tentacles of S. oualaniensis. These observations are consistent with the hypothesis that the tentacles separate gradually then rupture at the “wrist” (presumptive carpus), and argue against the possibility of prey capture by the fused tentacles.