Ontogeny of digestion in Daphnia: implications for the effectiveness of algal defenses

Abstract

Models of feeding and digestion predict that increased body size should result in longer gut passage time and improved assimilation efficiency. We examined the implications of digestion theory for size-structured interactions in a generalist zooplankton herbivore by studying the relationships between body size, ingestion rate, gut passage time (GPT), assimilation efficiency (AE), and growth rate in a clone of Daphnia pulex feeding on seven taxa of green algae that differed in digestibility. We also tested the effect of varying food concentration on GPT and AE while keeping body size constant. Food quality varied markedly among algal taxa, with mean juvenile growth rates at high food concentrations (1-2 mg/L) ranging from 0.10 to 0.61 d(-1). Juvenile growth rate for high food concentrations was highly correlated with juvenile AE (r2 = 0.96), verifying the importance of digestibility for food quality. AE, measured with 14C-labeled algae, increased with increasing age and body size for each of four digestion-resistant taxa but did not vary with age and body size for three readily digested algae. GPT decreased with decreasing body size, supporting the hypothesis that shorter GPT in juveniles leads to lower AE for digestion-resistant resources. Lower food concentrations led to increased GPT and improved AE for juveniles feeding on two digestion-resistant algae, providing further support for a role of longer gut retention in overcoming digestion defenses. The results suggest that increased abundance of digestion-resistant food will lead to growth and recruitment bottlenecks for juvenile herbivores, but that the effectiveness of digestion defenses will be decreased when large-bodied grazers predominate and when low food concentrations result in longer gut passage times. Gut processing constraints may favor either high concentrations of slow-growing, digestion-resistant resources or low concentrations of fast-growing, undefended resources.