Ontogenetic changes of the water status and accumulated soluble compounds in developing and ripening mume (Prunus mume) fruit measured by 1H-NMR analysis

Abstract

The physiological changes of intact mume (Prunus mume Sieb. et Zucc. cv. Rinshu) fruit tissues were examined by measuring the physical states of cell-associated water in the fruit tissues with developing and ripening using 1H-NMR spectroscopy. We found that the water molecules in mume fruit tissues existed in several different compartments with different mobilities. Additionally, spectral recovery in the water proton indicated reverse relationships between the pericarps and seeds at the immature and mature stages. In the pericarp tissues, the longest T1 and longer T2 markedly increased, while those in the seeds decreased. From these results, the change in the water status with growth stage had reverse trajectories in the pericarp and seed of the fruit. In the pericarp tissues, both water uptake and dry weight prominently increased with ripening. The epidermis and inner parenchymal cells of the pericarp tissues remarkably enlarged as a sigmoidal growth curve. Membrane permeability, indicating a loss of membrane integrity, increased in the pericarp tissues. The elongation in the fully vacuolated cells and changes in the membrane permeability in the pericarp tissues with ripening correlated to the longest T1. In contrast, the high mobility of water in the seeds began to decrease with maturation, while oil began to accumulate. Thus, the mobility of water, as analyzed in this study, is considered to reflect the results of physiological changes such as cellular heterogeneity and spatial arrangements both in the pericarp and in seed tissues for mume fruit with development and ripening.