Ontogenesis of F-actin in hair cells.

Abstract

This report describes the ontogenesis of cochlear stereocilia using scanning electron microscopy for analysis of cilia appearance, and fluorescence microscopy of phalloidin, a label for F-actin, to determine the maturation of the cilia framework. Surface and frozen-sectioned preparations of the otic capsule were obtained from several stages of rat pup development beginning at the 16th gestational day and at various stages until adulthood. In the earliest stage investigated, strong fluorescence labeling was visible on the apical part of Kölliker's organ, revealing a reticular outline of cell junctions. Hair cells started to differentiate at the 18th day of gestation from cells within the primordial receptor area. Phalloidin labeling revealed a sequential appearance of F-actin as the hair cells differentiated from the cells with the Kölliker's organ. The differentiation of receptor cells occurred first with the appearance of a junctional complex between the hair cell and the surrounding cells. Then a cuticular plate appeared followed by the progressive emergence of stereocilia. The F-actin labeling also revealed a progressive differentiation of receptor cells from the cochlear base to its apex. There was also an inner to outer hair cell developmental gradient of label. Inner hair cells developed stereocilia before outer hair cells. The third row of outer hair cells was the last to acquire stereocilia. The adult pattern of stereocilia was reached around the 6th postnatal day. We conclude that the appearance of actin filaments in developing receptor cells and the emergence of stereocilia can be regraded as markers for correlating function and other structural differentiation.