Abstract
Recently, several light microscopical approaches, such as point spread function (PSF) engineering, Spectral Precision Distance Microscopy (SPDM) and related methods have demonstrated the feasibility of measurements considerably beyond the conventional Abbe-Limit of optical resolution in far field light microscopy. The Heidelberg Spatially Modulated Illumination (SMI) microscope is based on the generation of a standing wave light field in the direction of the optical axis of the system. The theoretical PSF is obtained by the manipulation of the sin2-shaped illumination PSF and the epifluorescent detection PSF. Here, we report a software method to obtain online visualization of the axial light distribution of any object detected, developed using Microsoft Visual C++ and based on Windows NT. This strongly facilitates routine application of SMI Microscopy.© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
