Abstract
Biodegradation of organic compounds by natural attenuation or bioaugmentation is widely used for removal of unwanted chemicals from the environment. The essential component of remediation technologies is monitoring of a contaminant levels. Although the analytical methods of gas and liquid chromatography are available, they are time-, labour- and resource-demanding, placing limitations on the number of samples that can be analysed at a time. Furthermore, these methods cannot be easily adapted for in situ measurements. Biosensors can be used as an alternative or complement to these conventional techniques. Biosensors are based on a biological component coupled to a transducer, which translates the interaction between an analyte and a biocomponent into a signal that can be processed. Application of biosensors in monitoring of environmental contaminants is promising owing to their sensitivity, low costs, user-friendliness and adaptability for in situ measurements. In this contribution, we describe development of haloalkane dehalogenase-based biosensors and their application for detection of halogenated hydrocarbons in the environmental samples.
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