Abstract
Intensified bioprocess development requires parallelized medium- to high-throughput experimentation with high on- and offline data density across all early scales of the development trajectory from microtiter plate via shake flask to lab-scale reactor. We developed a widespread measurement principle for intermediate scales, respirometry, into a parallelized oxygen transfer rate measurement device that could accurately record common process development-relevant effects such as acetate formation, diauxic growth, and nutrient limitations. The device was further equipped with dissolved oxygen measurement capability and sampling ports that allowed repetitive monoseptic sample withdrawal without disturbing the cultivation. Optimization of the operating parameters lead to k(L) a values of up to 160 h(-1) and corresponding oxygen transfer rates of 1 g L(-1) h(-1) for cultivation volumes of 50 mL.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
