Abstract
screened by PCR analysis with primers specific for 6 carbapenemase genes. Amplification products were sequenced to determine the gene present. Results: Twelve of the 15 patients studied were hospitalizated at the ICU. The most frequent sites of isolation were the respiratory tract (16) and the blood (11). With the exception of colistin (0% resistance) there were no antibiotics with good activity against these isolates. Of the other antibiotics tested, tigecycline showed the best activity with an MIC90 value of 2 mg/L. The genotypic study revealed that the same strain was responsible for all the infections. All the isolates harboured the bla-OXA-51—like gene and the 6 of them chosen to sequence the gene were identical and 100% homologous with the bla-OXA-66 gene. PCR showed that the insertion sequence ISAba1 was present upstream of the oxacillinase gene in all the isolates. Conclusion: a) Molecular typing revealed that the outbreak detected in our hospital was due to a single A. baumannii clonal group. b) The epidemic strain of A. baumannii produces an OXA-66-ISAba1 carbapenem-hydrolyzing oxacillinase. c) Even though the outbreak was controlled, and the number of isolates decreased significantly, the clone responsible of the outbreak persisted at the hospital during the following year.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
