Abstract
This study described a physiologically relevant on-line electrochemical method for simultaneous monitoring of glucose and l-lactate in vivo with good sensitivity and selectivity. The analytical system that constructed by dual-enzyme biosensor was based on a novel and effective graphene hybrids with the on-line microdialysis system, which made the continuous and simultaneous monitoring of striatum glucose and l-lactate in brain of freely moving rats possible. The dual-enzyme biosensor was accomplished by using oxidases as the specific sensing unit and the UV-assisted synthesized Graphene/ZnO@Pd as the electrocatalyst. Transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray and X-ray photoelectron spectroscopy confirmed the denoted Pd nanoparticles (NPs) on the Graphene/ZnO composites. The analytical system showed good linearity for glucose and l-lactate concentrations from 20μM to 500μM with detection limit of 2.39μM and 2.52μM respectively. After post-calibration, the basal level concentrations of glucose and l-lactate in the microdialysate from the striatum of freely moving rats were calculated to be 0.323±0.021mM and 0.501mM±0.057mM (mean±s.d., n=3). Moreover, the dual oxidase/Graphene/ZnO@Pd based biosensors suffered from little cross-talk and exhibit a good stability and reproducibility.
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