Abstract
On-line detection of bacterial adhesion in a shear flow gradient was measured with an engineered bioluminescent bacterium. Parallel plate flow cells showed colonization by a Pseudomonas fluorescens strain containing the Vibrio fisheri (lux) bioluminescent operon. When induced by sodium salicylate, bulk phase and sessile cells were assayed quantitatively with an ammeter-photomultiplier-fiber optic system. The detection limit in a shear force gradient was 2×10 5 attached cells·cm −2 on glass coupons. Light production was found to correlate with biofilm lipid synthesis on a per unit area basis (1- 14C-acetate incorporation). Light correlated directly with sessile bacterial acridine orange direct counts in the range of 10 5–10 7 cells·cm −2, providing on-line detection of both biofilm and bulk phase biomass and specific metabolic activity when induced.
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