Abstract
A one-step triggered branched DNA nanostrucuture strategy was proposed for ultra-sensitive detection of microRNA (miRNA) based on two levels of hybridization chain reaction (HCR). The branched DNA nanostrucuture was produced through two HCR amplification procedures initiated by the target miRNA. After insertion of methylene blue (MB) into the base pairs, the concentration of miRNA can be obtained with a linear relationship from 10 fM to 50 pM by detecting the electrochemical signal of MB. The sensitivity for miRNA was significantly improved by HCR amplification, and the limit of detection was 2 fM. The proposed electrochemical biosensing strategy provides good reliability and ultra-high sensitivity as well as a satisfactory result for human serum sample. In addition, the one-step strategy greatly simplifies the operation step and reduce the systematic error, showing potential for real sample detection.
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