One-Pot Synthesis of Nucleoside-Templated Fluorescent Silver Nanoparticles and Gold Nanoparticles.

Furong Nie,Jun Ai,Lu Ga

doi:10.1021/acsomega.9b00701

Abstract

In this study, a simple one-pot method was proposed to synthesize water-soluble nucleoside-templated fluorescent silver nanoparticles (Ag NPs) and gold nanoparticles (Au NPs). The nucleoside-templated fluorescent Ag NPs and Au NPs were further characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and fluorescence spectroscopy (FLS). The effects of the molar ratio of reactants, reaction environment, and nucleotides on the synthesis of Ag NPs and Au NPs were also discussed. The results showed that nucleoside and ascorbic acid acted as a stabilizer and reductant, respectively, in the synthesis of Ag NPs and Au NPs, while citrate buffer acted as both a pH regulator and reductant. The synthesized nucleoside-templated fluorescent Ag NPs and Au NPs have good fluorescence stability and easy water solubility. In this study, a simple one-pot method was proposed to synthesize water-soluble nucleoside-templated fluorescent silver nanoparticles (Ag NPs) and gold nanoparticles (Au NPs).

Highlights

In recent years, fluorescent noble metal nanomaterials stabilized by nucleosides have become one of the most popular fluorescent nanomaterials due to their stable photochemical properties, adjustable fluorescence spectra, and good biocompatibility.[1−3] With the rapid development of nanoscience and nanotechnology, nucleosides are not confined to some surface functions
Many studies have reported that nucleosides are excellent templates for the preparation of fluorescent nanomaterials, which broaden the methods for the preparation of nanomaterials and promote biochemical analysis.[1,4−6] At present, the main research areas are as follows: quantum dots prepared with nucleosides as templates, but their wide application is inevitably hindered by the toxic side effects of quantum dots themselves;[7,8] fluorescent copper nanoparticles prepared with single/double chains as templates have gradually attracted the attention of researchers in the field of biochemistry, but the problems of poor optical stability and stringent buffer solution requirements are serious.[9,10]
Figure 2a−h showed that the nucleoside-templated fluorescent Ag NPs and Au NPs achieved the maximum fluorescence intensity under a heating condition for 3 and 4 h, respectively

Summary

INTRODUCTION

Fluorescent noble metal nanomaterials stabilized by nucleosides have become one of the most popular fluorescent nanomaterials due to their stable photochemical properties, adjustable fluorescence spectra, and good biocompatibility.[1−3] With the rapid development of nanoscience and nanotechnology, nucleosides are not confined to some surface functions. Many studies have reported that nucleosides are excellent templates (scaffolds) for the preparation of fluorescent nanomaterials, which broaden the methods for the preparation of nanomaterials and promote biochemical analysis.[1,4−6] At present, the main research areas are as follows: quantum dots prepared with nucleosides as templates, but their wide application is inevitably hindered by the toxic side effects of quantum dots themselves;[7,8] fluorescent copper nanoparticles prepared with single/double chains as templates have gradually attracted the attention of researchers in the field of biochemistry, but the problems of poor optical stability and stringent buffer solution requirements are serious.[9,10] The application of fluorescent silver nanoclusters and gold nanoclusters with nucleoside stabilization have been studied extensively. The concentration of various nucleotide was different (final concentration: 5 mM), and the Au NPs reacted in a water bath at 90 °C for 4 h

RESULTS AND DISCUSSION

CONCLUSIONS

■ ACKNOWLEDGMENTS

■ REFERENCES