Abstract
Aldehyde-functionalized mesoporous silica-Fe3O4 nanocomposites, one-pot synthesized by co-condensation of tetraethylorthosilicate and trimethoxysilylpropanal in presence of triblock copolymer of Pluronic P123, NaCl and Fe3O4 nanoparticles in the neutral solution, were characterized by SAXS, XRD, TEM, nitrogen sorption, FT-IR, TG, elementary analysis and magnetic susceptibility measurements, and investigated as the supports for covalent immobilization of penicillin G acylase (PGA). The results show that paramagnetic Fe3O4 nanoparticles were embedded among the cannular mesoporous silica layers and the aldehyde groups were condensed on the surface of mesoporous silica. PGA was covalently immobilized on these paramagnetic nanocomposites via the reaction to produce Schiff’s base between the free amino groups of lysine residues of PGA and the aldehyde groups on the surface of these nanocomposites. PGA immobilized on these paramagnetic nanocomposites had better operational stability and was easily recycled by an external magnetic field. The immobilized PGA had the initial activity of 6231Ug−1 and the operational stability of 91.0% of the initial activity after recycled for 10 times.
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