One year study of aerobic bacterial profile and antimicrobial susceptibility pattern of pus samples

Abstract

Pyogenic wound infection is one of the major cause of morbidity. The pace at which the bacterial isolates develop drug resistance is far exceeding the rate of discovery of newer drugs and thus highlights the need for conducting periodic studies to determine their antimicrobial susceptibility patterns. This study was conducted from June 2015 to May 2016, in Santhiram medical college and general hospital. Pus samples submitted to Microbiology department were processed and identified using standard protocols. Antimicrobial testing of all isolates was performed by Kirby- Bauer’s disc diffusion method as per CLSI guidelines. In our study a total of 490 pus samples were received, of which 279 (56.9%) were culture positive. Gram negative bacilli (78%) outnumbered Gram positive cocci (22%). Majority of samples were from Surgical departments (94.6%). E.coli (29%) was the commonest organism isolated followed by klebsiella spp. (19.7%), Staphylococcus aureus (15.4%).All Staphycoccal isolates were susceptible to Linezolid, Teicoplanin, Vancomycin where as Gram negative bacilli were susceptible to Imipenem (96.7%), Piperacillin-tazobactum (84.7%), Gentamicin (62.2%), Cefeperazone sulbactum (57.6%). Among Pseudomonas isolates, Imepenem (97.2%), Piperacillin-tazobactum (94.3%), were effective drugs. Microbiological analysis and antibiogram of pus isolates can serve as a useful tool for appropriate and judicial use of antibiotics and thus minimizing the evolution of drug resistance strains in future.