One-year stability of resin–dentin bonds created with a hydrophobic ethanol-wet bonding technique

Abstract

Dentin bonding performed with hydrophobic resins using ethanol-wet bonding should be less susceptible to degradation but this hypothesis has never been validated. Objectives This in vitro study evaluated stability of resin–dentin bonds created with an experimental three-step BisGMA/TEGDMA hydrophobic adhesive or a three-step hydrophilic adhesive after one year of accelerated aging in artificial saliva. Methods Flat surfaces in mid-coronal dentin were obtained from 45 sound human molars and randomly divided into three groups ( n = 15): an experimental three-step BisGMA/TEGDMA hydrophobic adhesive applied to ethanol (ethanol-wet bonding—GI) or water-saturated dentin (water-wet bonding—GII) and Adper Scotchbond Multi-Purpose [MP—GIII] applied, according to manufacturer instructions, to water-saturated dentin. Resin composite crowns were incrementally formed and light-cured to approximately 5 mm in height. Bonded specimens were stored in artificial saliva at 37 °C for 24 h and sectioned into sticks. They were subjected to microtensile bond test and TEM analysis immediately and after one year. Data were analyzed with two-way ANOVA and Tukey tests. Results MP exhibited significant reduction in microtensile bond strength after aging (24 h: 40.6 ± 2.5 a; one year: 27.5 ± 3.3 b; in MPa). Hybrid layer degradation was evident in all specimens examined by TEM. The hydrophobic adhesive with ethanol-wet bonding preserved bond strength (24 h: 43.7 ± 7.4 a; one year: 39.8 ± 2.7 a) and hybrid layer integrity, with the latter demonstrating intact collagen fibrils and wide interfibrillar spaces. Significance Coaxing hydrophobic resins into acid-etched dentin using ethanol-wet bonding preserves resin–dentin bond integrity without the adjunctive use of MMPs inhibitors and warrants further biocompatibility and patient safety's studies and clinical testing.