Abstract
A one-step signal amplified lateral flow strip (LFS) biosensor has been developed for ultrasensitive and on-site visual detection of bisphenol A (BPA). This signal amplified LFS was based on the dual labeling using different-sized gold nanoparticles (Duo-LFS). This Duo-LFS could achieve BPA detection with 0.5ng/mL as the visual sensitivity by naked eye observation and with 0.076ng/mL as the limit of detection (LOD) for semi-quantitative detection by software analysis, which is at least 10-fold improvement of the sensitivity of traditional LFS based methods. This one-step signal amplified lateral flow strip biosensor and related signal enhancement method could be adopted as a potential generous technique for all LFS-based detection methods.
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