Abstract
‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.
Highlights
BRIEF REPORTOne-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
Tomato torrado virus (ToTV) belongs to the genus Torradovirus in the family Secoviridae [1]
Symptoms of tomato torrado virus (ToTV) infection in tomato begin with the yellowing of the leaflet base, which develops into necrosis of the whole plant, including fruits, often causing its death [1, 2]
Summary
One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus. Received: 16 October 2015 / Accepted: 26 January 2016 / Published online: 18 February 2016 Ó The Author(s) 2016. This article is published with open access at Springerlink.com. Abstract ‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. A one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive
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