Abstract
A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Capsicum chlorosis virus (CaCV) was developed and optimized. The RT-LAMP assay was completed within 60 min using isothermal conditions and four primers targeting the CaCV nucleocapsid protein gene. The sensitivity of this assay was higher than that of a reverse transcription polymerase chain reaction using total RNA of infected Phalaenopsis sp. The assay was highly specific allowing CaCV to be differentiated from other orchid-infecting viruses. The result of the assay can be visualized under UV with the addition of SYBR Green I dye to the end product directly in the tube. This assay is a useful tool for the detection of CaCV infected orchids due to its rapidity, simplicity, accuracy and sensitivity.
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