Abstract
This paper describes a single-step green synthesis of extracellular silver nanoparticles (AgNPs) through an in-situ bio-reduction of aqueous solution of silver nitrate using Psidium guajava L. UV–vis exhibits a characteristic surface plasmon resonance peak at around 487nm. XRD analysis showed crystalline and face-centered cubic geometry of synthesized AgNPs. TEM results revealed stable and spherical particles with a mean diameter size of ~60nm. Fourier transform infrared spectroscopy showed that the nanoparticles were capped with plant bioactive molecules. Phytosynthesized AgNPs showed broad-spectrum anti-microbial activity against Gram-positive, Gram-negative human pathogenic bacteria, and fungi. AgNPs showed prominent ability to inhibit the biofilms formed by Staphylococcus aureus, Escherichia coli and Candida albicans in a laboratory condition through crystal violet assay. The results suggest AgNPs as a promising candidate for the design of an effective antimicrobial and anti-biofilm agent in biomedical field.
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