Abstract
In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H2O2 and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0.006mM for dynamic response range of 0.01–0.6mM. The calculated higher value of Vmax (7.2 × 10−7Ms−1) along with lower Km (0.012mM) corroborate enhanced the peroxidase-like activity of E-GQDs. Study introduces a cheap and widely available raw material for the electrochemical synthesis of graphene quantum dots with commendable enzyme mimetic activity which may have a huge impact in developing calorimetric bioanalysis systems.
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