Abstract
Oligonucleotides containing the 5′ termini of adenovirus 2 mRNA are selectively retained on columns of dihydroxyboryl cellulose. When total late adenovirus 2 mRNA was treated with RNAase T1, a single 5′ terminal oligonucleotide was isolated, although in several states of methylation. This oligonucleotide has the general structure m 7 GS 5′ ppp 5′ A ★ mCmU(C 4,U 3)G. Since at least twelve individual species of mRNA must be present late after infection, this finding was unexpected and its significance is discussed.
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