One-pot treatment of Saccharophagus degradans for polyhydroxyalkanoate production from brown seaweed

Abstract

The conventional production of polyhydroxyalkanoate (PHA) from waste biomass requires a pretreatment step (acid or alkali) for reducing sugar extraction, followed by bacterial fermentation. This study aims to find a greener approach for PHA production from brown seaweed. Saccharophagus degradans can be a promising bacterium for simultaneous reducing sugar and PHA production, bypassing the need for a pretreatment step. Cell retention cultures of S. degradans in membrane bioreactor resulted in approximately 4- and 3-fold higher PHA concentrations than batch cultures using glucose and seaweed as carbon sources, respectively. X-ray diffraction, Fourier transform infrared spectroscopy, and nuclear magnetic resonance results revealed identical peaks for the resulting PHA and standard poly(3-hydroxybutyrate). The developed one step process using cell retention culture of S. degradans could be a beneficial process for scalable and sustainable PHA production.