One-pot synthesized copper-imidazole-2-carboxaldehyde complex material with oxidase-like activity for the colorimetric detection of glutathione and ascorbic acid

Abstract

Due to the copper (Cu) active sites, its complexes with oxidase-like activity have superior catalytic properties, which can catalyze a series of specific substrates like 3,3′,5,5′-tetramethylbenzidine (TMB), producing colorimetric reactions for the detection of different reducing small-molecule compounds. Attribute to the competitive coordination effects between water molecules and central Cu ions, most of the Cu complexes can hardly be used in the pure aqueous reaction system. In this study, a Cu-based material (Cu-imidazole-2-carboxaldehyde, Cu-ICA) was prepared using copper ions and ICA through a one-step process in the water solution. After the morphology of the material being characterized, the mimetic enzyme behavior of the Cu-ICA was demonstrated through the TMB oxidation. Compared to the other reported oxidase-like mimics, Cu-ICA has better aqueous stability and oxidase-like activity, and shows a higher vmax. Furthermore, basing on the oxidase-like activity of Cu-ICA, a colorimetric method was developed for the ascorbic acid and glutathione detections with linear ranges of 0.5–5 μM and 0.5–4 μM, and limit of detection of 0.1304 μM and 0.097 μM, respectively. Owing to its excellent aqueous stability and oxidase-like activity, Cu-ICA has bright application prospects in the analysis of reducing small-molecule compounds.