One‐Pot Synthesis of Orange Emissive Carbon Dots Specific for Staining of Mitochondria in both Cancer and Non‐Cancer Cell Lines

Abstract

Mitochondria are recognized as essential organelles that form a dynamic network responsible for energy production, calcium homeostasis, and cell signalling for cell metabolism. Dysfunction in mitochondria results in many diseases, such as Alzheimer’s, Muscular dystrophy, Diabetes, and Parkinson’s. As a result, it is of great significance to directly visualize mitochondria inside cellular environment for therapeutic target. Carbon dots (CDs) are one of the probes with low toxicity and high biocompatibility that have been utilized for mitochondrial visualization. However, till date, CDs are synthesized using multi‐step synthesis and most of them emit in the blue to green region thus limiting their application in bioimaging. Herein, we synthesized orange emissive mitochondria‐specific CDs via a one‐pot method using 3‐(carboxypropyl) triphenylphosphonium bromide (TPP) and citric acid as precursors. Synthesized CDs have excellent photoluminescence properties to stain and image mitochondria in different cancerous and non‐cancerous mammalian cells.