One-Pot Reducing Agent-Free Synthesis of Silver Nanoparticles/Nitrocellulose Composite Surface Coating with Antimicrobial and Antibiofilm Activities.

K G U R Kumarasinghe,W C H Silva,M Shimomura,P M Jayaweera,M D A Fernando,L Palliyaguru,T D C P Gunasekara,S S N Fernando,P S Jayawardena,Yu Chang Tyan

doi:10.1155/2021/6666642

K G U R Kumarasinghe, W C H Silva + Show 8 more

Open Access

https://doi.org/10.1155/2021/6666642

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Abstract

Nitrocellulose with silver nanoparticle (AgNP/NC) composite was prepared in situ using Ag(CH3CO2) and nitrocellulose without any reducing agent. The composite materials synthesized were spray coated onto glass substrates to obtain thin films. The AgNPs/NC composites were characterized by ultraviolet-visible, Fourier transform infrared, X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The antimicrobial activity of AgNPs/NC composite was investigated by tube method and time-kill kinetic studies against three microbial species, including Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and Candida albicans (ATCC 10231). The antibiofilm activities were qualitatively determined against all three organisms. Prepared AgNPs/NC films exhibited good antimicrobial activity and significant inhibition of biofilm development against all three microbial species. The effective dispersion of AgNPs/NC in biofilm was responsible for the significant antibiofilm activity of the prepared material. The reported AgNPs/NC composite can be used as coating additive in bacteriocidal paint which can be applied onto surfaces such as in healthcare environments.

Highlights

There are increasing reports of patients with hospital acquired infections and emergence of multidrug-resistant microbial pathogens leading to a higher mortality, morbidity, and increased length of hospital stay [1,2,3]
Results clearly indicate that incorporation of 10 wt% AgNPs into the NC matrix does not negatively affect the chemical and physical properties of NC
The average Ag particle size is around 25 ± 5 nm, and the prepared surface coatings on glass substrates are highly stable under ambient conditions

Summary

Introduction

There are increasing reports of patients with hospital acquired infections and emergence of multidrug-resistant microbial pathogens leading to a higher mortality, morbidity, and increased length of hospital stay [1,2,3]. Microorganisms that attach to the surfaces in the healthcare environment, such as hospital beds and walls, can cause acute and chronic infections [4, 5]. These bacteria or yeast form multicellular biofilms in which the microorganisms are protected from killing by host defenses and antibiotics [6]. Contamination of surfaces such as walls and beds especially in the hospital setting can transmit infection via hands. The development of healthcare-related products, such as textiles, BioMed Research International medical devices, and surface coatings, with antimicrobial and antibiofilm properties can significantly reduce transmission of microbial infections [11,12,13,14]

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