One-pot hydrothermal green synthesis of Polygala tenuifolia mediated graphene quantum dots for acetylcholine esterase inhibitory activity

Abstract

The present paper reports the synthesis of Donepezil hydrochloride functionalized Graphene quantum dots (DO-ptGQD) for the cure of Alzheimer disease. Graphene quantum dots were synthesized through root extract of Polygala tenuifolia by using one-pot hydrothermal synthesis method. Using UV/Visible spectroscopy, FTIR, SEM, TEM and XPS methods, the synthesized ptGQD was evaluated. Further the formulated DO-ptGQD was evaluated by SEM, TEM, FTIR, XPS techniques and was further evaluated for the encapsulation study, in-vitro study and various in-vivo models. Through behavioural model (Radial arm maze and Water morris maze assay) the learning and memory ability of the formulations was evaluated and the outcomes indicate that the DO-ptGQD markedly decreases the transmission latency to get in the baited arm in 12.19 ± 0.39 s or to the hidden stage in 15.37 ± 0.45 in 7 days. DO-ptGQD show more percentage inhibition of AchE enzyme that is 86.23 ± 1.28 as likened with the drug and ptGQD. DO-ptGQD considerably increases the protein and glutathione level and decreases the nitric oxide and lipid peroxide level. The Histopathological image indicates that DO-ptGQD treats the disorganization of the cell and show more preservation of small pyramidal cells as compared with the other formulations. In conclusion, DO-ptGQD was safe and effective drug delivery system for the treatment of Alzheimer disease and also the root extract of Polygala tenuifolia provide synergistic effect for the treatment of disease.