Abstract
Gold nanoparticles have been successfully synthesized using aqueous leaf extract of Piper betle In this extracellular synthesis, after exposing of metal ions to betel leaf extract, reduction leads into their metallic state and these are stabilized by the biomolecules present in leaf extract, where extract are being used as both reducing as well as stabilizing agents at ambient condition. Gold (AuNPs) nanoparticles are characterized by UV-Vis, FESEM, HRTEM and XRD measurements. Synthesized gold nanoparticles are mostly spherical in shape with diameter ~ 30-50 nm. Antibacterial activities of the synthesized nanoparticles are investigated against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive (Staphylococcus aureus and Bacillus thuringiensis) bacteria using the disc diffusion method. AuNPs show inhibition activity against P. aeruginosa andE. coli respectively nearly equivalent to the commercially available antibacterial drug e.g. Norfloxacin (Nx). The minimum inhibition concentration (MIC) results indicate that 36 μg/mL gold nanoparticles inhibit the growth of E. coli cells.
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