Abstract
AbstractTwo improved silver methods for fungi and Pneumocystis carinii that use a modified oxidizing agent are presented. The sections were first oxidized with a solution consisting of chromic, sulfuric, and glacial acetic acids for 1 min in the 60°C paraffin oven, thoroughly washed in running tapwater, and treated with sodium metabisulfite before they were impregnated in modified silver methenamine or ammoniacal silver nitrate solution. The sections were treated with sodium thiosulfate before and after gold chloride. They were counterstained with light green.This modified oxidation prevented staining of elastic and collagen fibers. The entire procedure was performed in approximately 40 min or less, depending on the temperature used in the silver impregnation. All fungi tested and P. carinii were stained in the same amount of time in both silver nitrate and gold chloride. (The J Histotechnol 15:307, 1992)
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