Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Cervical Cancer Cells Hela S3.

Jiajun Ni,Xiang Xu,Hualin Feng,Ting Ye,Kan Chen,Gongchu Li,Tingting Liu

doi:10.3390/md19100532

Abstract

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin produced by sponges. Our previous study demonstrated that genes encoding AVL enhanced the cytotoxic effect of oncolytic vaccinia virus (oncoVV) in a variety of cancer cells. In this study, the inhibitory effect of oncoVV-AVL on Hela S3 cervical cancer cells, a cell line with spheroidizing ability, was explored. The results showed that oncoVV-AVL could inhibit Hela S3 cells growth both in vivo and in vitro. Further investigation revealed that AVL increased the virus replication, promote the expression of OASL protein and stimulated the activation of Raf in Hela S3 cells. This study may provide insight into a novel way for the utilization of lection AVL.

Highlights

Vaccinia virus is a large, enveloped, double-stranded DNA virus with a linear genome approximately 190 kb in length
We investigated the inhibitory effect of oncolytic vaccinia virus (oncoVV)-Aphrocallistes vastus lectin (AVL) on cervical cancer cell line Hela S3, a tumor cell with spheroidizing characteristics, and further analyzed the underlying mechanism
The results indicated that sorafenib that AVL enhanced virus replication by activating

Summary

Introduction

Vaccinia virus is a large, enveloped, double-stranded DNA virus with a linear genome approximately 190 kb in length. It has been widely used in the eradication of smallpox [1]. Oncolytic vaccinia virus harboring therapeutic genes has been applied to lyse tumor cells directly [2,3,4]. Several oncolytic vaccinia viruses derived from Wyeth, Western. The strategies to improve the oncolytic efficacy of vaccinia vectors have become the focus of research. The role of the marine environment in the development of anticancer drugs has been widely reviewed. The compounds derived from marine organisms may be an inspiring tool to develop new anticancer agents [8]. Lectins are widely distributed in marine bioresources, such as marine cyanobacteria, algae, invertebrate animals and fish

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