Oncolytic effect of Newcastle disease virus strain D417 On small cell lung cancer in vitro

Abstract

Objective To study the cytolytic effect of D417 strain of Newcastle disease virus (NDV)on small cell lung cancer cell line NCI-H446 and normal cell line HFL-1. Methods Death rates of NCI-H446 and HFL-1 cells were measured through methyl thiazolyl tetrazolium eolorimetric as-say after the cells were infected by NDV strain D417 in vitro.Pathological changes of NCI-H446 cells were observed under light and electron microscopes. Results NDV strain IMl7 killed NCI-H446 cells.and the eell death rate was positively correlated with viral dose and incubation time.Strain D417 also kill-ed HFL-l cells,but the death rate of infected HFL-1 cells was lower than that of NCI-H446 cells under the same condition.After strain D417 infection.the cytopathogenic effect and fusion of NCI-H446 cells were found under the light microscope,apoptosises were found by electron microscopy. Conclusions NDV strain D417 has the ability to kill small cell lung cancer cells and a relatively less influence on nor-mal cells. Key words: Newcastle disease virus; Carcinoma,small cell; In vitro