Oncolytic adenovirus for imaging and treatment of pancreatic cancer

Abstract

s / Pancreatology 13 (2013) e1–e94 e22 Methods:We assembled an islet isolation laboratory within a large operating room where the pancreatectomies were performed. This consisted of a hooded workstation (Labconco Class II Type A2), a centrifuge (Beckman Coulter Allegra 25R), and an islet isolation system (BiorepRicordi Islet Isolator) comparable to thoseused in thestandard laboratorysetting.Apancreatectomy was performed with immediate isolation of the islets within the operating room. As soon as the islets were suspended in the final preparation solution, they were infused into the portal venous system. The gastrojejunostomy and hepaticojejunostomywereperformedduring the intraoperative islet isolation. Results: We report 8 patients who underwent successful intraoperative islet isolation with reinfusion over an 8-month period (8/2011 – 4/2012). Mean total operative time was 8.8 hrs (range 7.3 13 hrs). Mean islet equivalent (IEQ) yield was 707,235 islets (range 250,000-1,400,000 islets). Of these, 88% (7/8) are producing C peptide. Conclusion:We describe the first report of a totally intraoperative islet isolation series, which eliminates the time, cost and risk of processing the gland in a separate remote laboratory. Intraoperative islet isolation resulted in comparable islet yield to that described with utilization of a remote laboratory. The transplanted islets using this novel technique had a good functional outcome.