Abstract
The initiation of the DNA replication cycle in Escherichia coli requires protein synthesis. Marunouchi & Messer (1973) have hypothesized that an additional protein synthesis step is required for the replication of the terminal segment of the chromosome, and that replication of this segment is a prerequisite for subsequent cell division. We have not confirmed the existence of a unique terminal segment using a protocol designed to label the hypothesized segment with [ 3H]dThd † † dThd, deoxythymidine . Our protocol avoids the increased incorporation of [ 3H]dThd into DNA caused by abrupt increases in temperature, a complication implicit in the technique of Marunouchi & Messer (1973). Treatment with nalidixic acid (an inhibitor of semiconservative DNA synthesis) in sufficient concentration to prevent replication of the postulated terminal segment prevents cell division but also causes loss of viability. This makes it difficult to correlate the effect of nalidixic acid on cell division with DNA synthesis inhibition alone.
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