Abstract
AbstractIn this article we revisit the synthetic methodology for oligonucleotides interconnected via pyrophosphates that relies on reactivity of phosphorodiamidous anhydrides. We have found this method, previously published by Ahmadibeni and Parang (2007), to be irreproducible in our hands. We synthesized successfully three pyrophosphate interconnected thymidine oligonucleotides, identical to the claimed structures in their article, via a method independently developed by us. Evaluation of the spectroscopic data of the newly synthesized products casts doubts on the viability of the Ahmadibeni–Parang approach. Therefore, we believe to report here the first successful synthesis of DNA oligomers interconnected via pyrophosphate linkages.
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