On the study of molecular markers of the genus <i>Picea</i> in Russia and Belarus

Abstract

Currently, degradation of spruce stands is observed in various regions of Russia, which is associated with the transformation of climatic and environmental conditions of the environment. To ensure effective reforestation and afforestation of spruce forests, it is necessary to study the features of the population-genetic structure of plantations in order to preserve the genetic resources of valuable forest-forming tree species. The purpose of this work is to review modern achievements in the study of molecular markers of the genus Picea in Russia and Belarus. To characterize the genetic diversity of spruce populations, 9 ISSR primers with a high level of polymorphism (86,6%) were selected: (GA)9T, (AC)8C, (CA)6RY, (CA)6RG, (CA)6(GT), (CA)6(AC), (AG)8T, (GA)8C and (AG)8YT. The analysis of the mh44 locus of mitochondrial DNA revealed 13 allelic variants and is suitable for studying the genetic diversity of intra- and inter-population genetic variability of spruce. Experimental work with the mitochondrial Nad1 gene has shown the possibility of its application for phylogeographic studies of spruce. The assessment of the genetic variability of microsatellite loci Pt63718, Pt26081, Pt71936 revealed 19 allelic variants and is also suitable for phylogeographic studies. These results are very valuable and can form the basis for studying and characterizing the genetic structure of spruce plantations.