Abstract
The effects of freezing, rate of freezing, filtration, preservatives, and the type of storage container on ammonia concentrations of stored seawater samples were investigated. Significant changes took place in samples stored without treatment. Ammonia increased when stored in glass, but decreased when stored in polyethylene containers. Quick freezing and slow freezing stabilized ammonia concentrations, although variability was increased; quick freezing had no advantage over slow freezing. Preservation with phenol at the same concentration used in the analytical method (0.4 g 100 ml−1) stabilized unfrozen samples for up to 2 weeks.
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