On the specificity of the two ribosomal binding sites: Studies with tetracycline

Abstract

The erroneous binding of isoleucyl-tRNA to ribosomes in response to poly U shows the same pattern of inhibition by tetracycline as the correct binding of phenylalanyl-tRNA, while the poly U directed binding of leucyl-tRNA is largely sensitive to tetracycline. It is concluded that the coding specificity of the peptidyl site on the ribosome is at least equivalent to that of the aminoacyl site.