On the role of rat liver cytosol in suppression of low-level chemiluminescence during nonenzymatic lipid peroxidation

Abstract

1. 1. The effect of normal rat liver cytosol on the level of Fe/ADP-ascorbate-induced lipid peroxidation in the total particulate fraction (mitochondria plus microsomes) has been studied. The intensity of lipid peroxidation was measured using chemiluminescence technique and malondialdehyde (MDA) formation. 2. 2. Dialysed cytosol significantly decreased the level of chemilurninescence, and to a much lesser extent, the rate of MDA production. 3. 3. Gel filtration on a Sephadex G-200 column led to appearance of at least three cytosolic fractions which suppressed the low-level chemilurninescence. 4. 4. The discovered components differed from each other by their molecular masses, kinetics of chemilurninescence inhibition and effects on intensity of MDA formation. 5. 5. The putative functional role of antioxidative defence factors from rat liver cytosol is discussed.